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National Biomedical Resource
for Advanced Electron Spin Resonance Spectroscopy

Our research is supported by grant 1R24GM146107 from the National Institute of General Medical Sciences (NIGMS), part of the National Institutes of Health.

  Key publications
Protein ESR
Articles:

Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis
M. Dong, V. Kathiresan, M. K. Fenwick, A. T. Torelli, Y. Zhang, J. D. Caranto, B. Dzikovski, A. Sharma, K. M. Lancaster, J. H. Freed, S. E. Ealick, B. M. Hoffman, and H. Lin.
Science 359, 1247-1250 (2018)
Supporting Information
<doi:10.1126/science.aao6595>
PMID: 29590073      PMCID: PMC6066404
 

Unique Structural Features of Membrane–Bound C–Terminal Domain Motifs Modulate Complexin Inhibitory Function
D. Snead, A. L. Lai, R. T. Wragg, D. A. Parisotto, T. F. Ramlall, J. S. Dittman, J. H. Freed, and D. Eliezer.
Front. Mol. Neurosci. 10, 154 (2017)
Supporting Information
<doi:10.3389/fnmol.2017.00154>
PMID: 28596722      PMCID: PMC5442187
 

Substrate–Dependent Cleavage Site Selection by Unconventional Radical S–Adenosylmethionine Enzymes in Diphthamide Biosynthesis
M. Dong, M. Horitani, B. Dzikovski, J. H. Freed, S. E. Ealick, B. M. Hoffman, and H. Lin.
J. Am. Chem. Soc. 139, 5680-5683 (2017)
Supporting Information
<doi:10.1021/jacs.7b01712>
PMID: 28383907      PMCID: PMC5664936
 

Organometallic Complex Formed by an Unconventional Radical S-Adenosylmethionine Enzyme
M. Dong, M. Horitani, B. Dzikovski, M. -E. Pandelia, C. Krebs, J. H. Freed, B. M. Hoffman, and H. Lin.
J. Am. Chem. Soc. 138, 9755-9758 (2016)
Supporting Information
<doi:10.1021/jacs.6b04155>
PMID: 27465315      PMCID: PMC5068486
 

A Fluorimetric Readout Reporting the Kinetics of Nucleotide-Induced Human Ribonucleotide Reductase Oligomerization
Y. Fu, H.-Y. Lin, S. Wisitpitthaya, W.A. Blessing and Y. Aye.
Chembiochem 15 2598-2604 (2014)
Supporting Information
<doi:10.1002/cbic.201402368>
PMID: 25256246      PMCID: PMC4271543
 

Uncoupling of Allosteric and Oligomeric Regulation in a Functional Hybrid Enzyme Constructed from Escherichia coli and Human Ribonucleotide Reductase
Y. Fu, M.J.C. Long, M. Rigney, S. Parvez, W.A. Blessing, Y. Aye.
Biochemistry 52 7050-7059 (2013)
Supporting Information
<doi:10.1021/bi400781z>
PMID: 24024562